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Elementar’s LiquiFace is a specialist solution providing high-performance carbon isotope analysis of liquids and separated compounds, with significant benefits in terms of versatility and ease of use.
Compound-specific isotope analysis of aqueous samples by liquid chromatography is highly advantageous for compound classes that are thermolabile, or require derivatisation prior to gas chromatography analysis. Samples such as carbohydrates, amino acids, alcohols, organic acids and peptides make ideal candidates for LC-IRMS, and the technique has been employed to great success in food and biogeochemistry applications.
LiquiFace works as an interface for the LC system to provide quantitative online conversion of organic carbon to CO2, prior to analysis by the IRMS system. Isotopic precision, accuracy and linearity is exceptional due to the highly efficient quantitative conversion of the organic carbon to CO2. This level of performance means that LiquiFace is able to cover a range of sample types with a complete independence upon sample concentration, making the analyst’s life even simpler.
100% quantitative conversion
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Robust and thermally stable micro-reactor
High-efficiency water separation
membrane for extraction of dissolved CO2 into helium carrier gas, with no isotopic fractionation
Onboard peristaltic pumps
that provide easily maintained flows of reaction reagents
Connection to any standard LC or IC pumping system
using finger-tight peek union connections
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valve and 25μl sample loop for performing bulk δ13C isotope analysis of aqueous samples
The LiquiFace can be connected to an HPLC unit to receive a sample liquid stream for conversion to CO2. Alternatively, the internal pump on the LiquiFace can be used to carry out direct injection of samples.
Peristaltic pumps in the LiquiFace are used to deliver reagents to the reaction site and not the HPLC pump, maximizing the user’s operational flexibility and choices. Direct injection to the LiquiFace means that users can perform both bulk isotopic and compound-specific analysis using the same system.
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LC-IRMS analysis of non-polar compounds do not need to undergo complex derivatization, as would be the case if the same sample was run via GC-IRMS. The removal of any need for derivatization on these compounds means that back calculations do not need to be performed on the eventual 13C results. In many cases, samples need only be diluted with pure water, filtered and then analyzed with the LiquiFace.
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When the LiquiFace is coupled to an Isoprime IRMS, the result is an exceptional system with an unparalleled tolerance for excess oxygen load. With the use of sodium persulfate as an oxidation agent, a large oxygen excess can enter the IRMS and damage the ionization filament.
The unique thorium coating of the isoprime precisION ionization filament results in a product lifespan measured in years, rather than months. This resistance to oxygen means that an online reduction furnace to remove excess oxygen is not required, reducing consumables and possible peak broadening.
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The LiquiFace has been completely designed with chromatography in mind. Once your sample is separated by the LC column, any detrimental effect of the interface on the chromatography is minimal. As such, the LiquiFace can be depended upon to provide the very best in chromatography performance for particularly complex separations.
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Conversion of the aqueous eluent to sample gas is performed in a low-temperature reactor at up to 100°C. Low-temperature conversion of the eluent allows our interface to analyze thermolabile samples, which would disintegrate at elevated temperatures.
The mixture of eluent and sample gases, generated in the LiquiFace heater, are cooled to room temperature and then separated in a gas exchanger. The remaining eluent goes into a waste line, while the sample gas passes through a water removal membrane into the IRMS.
The Agilent 1260 Infinity LC system, configured for analysis of constituent glucose, fructose and disaccharides, can be added for the detection of honey adulterations with C3 sugars.
The Agilent 1260 Refractive Index Detector can be used for simultaneous quantitative analysis, as well as isotopic ratio analysis.